Introduction: Cellular Structure and Function
All organisms are made of cells. Complex organisms (such as humans) are made up of collections of highly specialized cells, each with its own specific function. Different types of cells have evolved specific shapes and properties which help them do their jobs inside the organism.
Still Image:
Mouse embryonic stem cells with stained nuclei - image 1
These mouse embryonic stem cells have been treated with a stain that makes DNA fluoresce, causing nuclei to appear blue.
Pluripotent mouse embryonic stem cells were cultured using standard techiniques. For imaging, cells in suspension were transferred to small, sterile plexiglass chambers attached to a coverslip. For imaging, growth media was replaced with phosphate buffered saline containing DAPI. Cells were maintained on an inverted compound microscope in an environmental chamber kept at 37°C, 7.0% CO2. Images were taken with a 40x phase contrast objective, using fluorescent light, DAPI filter and a digital camera.